成年大鼠神经元和星形胶质细胞细胞周期蛋白表达的差异研究

目的比较研究成年大鼠细胞周期蛋白在神经元和星形胶质细胞的表达差异。方法应用免疫荧光和激光扫描共聚焦显微镜观察成年大鼠生理状态下大脑皮层或海马CA1区神经元和星形胶质细胞细胞周期素D1、E、A、B1、(CyclinD1、E、A、B1)的表达。结果成年大鼠海马CA1区和大脑皮层的神经元有Cyclin D1、E、A和B1的表达,细胞核和细胞浆均有表达,以胞核为主;星形胶质细胞也有上述细胞周期蛋白的表达但细胞数目较少,并且表达这些指标的星形胶质细胞多聚集在海马CA1区。结论成年大鼠大脑皮层和海马区的神经元和星形胶质细胞均表达细胞周期蛋白,而其在神经元的表达较星形胶质细胞更为普遍。

DIFFERENCE AND SIGNIFICANCE OF CYCLINS IN NEURONS AND ASTROCYTES OF ADULT RATS

Objective To study the difference of cyclins between neurons and astrocytes in adult rats.Methods The expressions of CyclinD1,E,A and B1 of neurons and astrocytes in cerebral cortex or hippocampal CA1 region of adult rats were detected by immunofluorescence and confocal microscopy.Results All cyclins were expressed in nucleus or/and cytoplasm of postmitotic neurons,mainly in the nucleus,of cerebral cortex or CA1 subfield of hippocampus;Whereas only a few astrocytes expressed cyclins in hippocampus and cerebral cortex and most of these astrocytes were located in hippocampal CA1 region.Conclusion Both neurons and astrocytes were expressed all cyclins in hippocampus and cerebral cortex of adult rats.Compared with that of the astrocytes,the expressions of cyclins in mature neurons were remarkable.