| 丙酮酸对肺炎克雷伯氏菌微氧发酵甘油产1,3-丙二醇的影响 |
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| 引用本文： | 王晓楠,王凤寰,吴家鑫,谭天伟. 丙酮酸对肺炎克雷伯氏菌微氧发酵甘油产1,3-丙二醇的影响[J]. 生物加工过程, 2010, 8(3): 23-27. DOI: 10.3969/j.issn.1672-3678.2010.03.005 |
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| 作者姓名： | 王晓楠 王凤寰 吴家鑫 谭天伟 |
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| 作者单位： | 北京化工大学,生命科学与技术学院,北京市生物加工过程重点实验室,北京,100029 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目，国家高技术研究发展计划(863 计划)资助项目，国家自然科学基金资助项目，北京市自然科学基金资助项目，北京市教育委员会共建项目 |
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| 摘 要： | 微氧条件下,考察肺炎克雷伯氏菌发酵生产1,3-丙二醇过程中柠檬酸和丙酮酸对发酵过程的影响。摇瓶实验结果表明:添加柠檬酸能抑制菌体生长和1,3-丙二醇合成;丙酮酸对菌体生长和1,3-丙二醇合成有一定的促进作用。5 L发酵罐批式发酵表明:补料培养基中加入8 g/L丙酮酸,1,3-丙二醇的产量提高了约10.8%,转化率提高了约4.4%,比生长速率提高了约10.8%。上述结果初步表明,强化能量的产生能够有效促进1,3-丙二醇的合成,可以利用分子生物学手段强化丙酮酸的产生以促进1,3-丙二醇的合成。
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| 关 键 词： | 丙酮酸 1,3-丙二醇 微氧发酵 |
Effect of pyruvate on microaerobic fermentation of 1,3-propanediol from glycerol by Klebsiella pnenmoniae |
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| WANG Xiao-nan,WANG Feng-huan,WU Jia-xin,TAN Tian-wei. Effect of pyruvate on microaerobic fermentation of 1,3-propanediol from glycerol by Klebsiella pnenmoniae[J]. Chinese Journal of Bioprocess Engineering, 2010, 8(3): 23-27. DOI: 10.3969/j.issn.1672-3678.2010.03.005 |
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| Authors: | WANG Xiao-nan WANG Feng-huan WU Jia-xin TAN Tian-wei |
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| Affiliation: | (Beijing Key Laboratory of Bioprocess,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China) |
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| Abstract: | Under microaerobic conditions,effects of citrate and pyruvate on 1,3-propanediol（1,3-PD） fermentation by Klebsiella pnenmoniae were studied.The experiments were carried out in shake flask.The results showed that the enhancement of cell growth and 1,3-PD synthesis was found by adding pyruvate,while the inhibition of those was found with the addition of citrate.Fed-batch fermentation was then studied in a 5 L fermentator by the addition of pyruvate into feeding.The final concentration of 1,3-PD increased about 10.8% with the convert ratio and the specific growth rate of 4.4% and 10.8%.Results showed that enhancement of 1,3-PD synthesis was improved by adding ATP. |
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| Keywords: | pyruvate 1 3-PD microaerobic fermentation |
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