Determination of urinary amino acids by means of glass capillary gas—liquid chromatography with alkali-flame ionisation detection and flame ionisation detection |
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| Authors: | Jasbir Chauhan Andre Darbre Roy F. Carlyle |
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| Affiliation: | Department of Biochemistry, King''s College London, Strand, London WC2R 2LS Great Britain;The Physiological Laboratory (A.M.T.E.), Fort Road, Gosport, Hants Great Britain |
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| Abstract: | The determination of amino acids as their N(O)-heptafluorobutyryl-isobutyl ester derivatives by glass capillary gas—liquid chromatography has been studied. Separations of amino acids obtained from insulin hydrolysate and human urine analysed with a flame ionisation detector, an alkali-flame ionisation detector and an electron-capture detector are shown. The quantitative results of urinary amino acids of one deep-sea diver are presented. |
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