Direct gene transfer inPetunia hybrida electroporated protoplasts: Evidence for co-transformation with a phosphoenolpyruvate carboxylase cDNA from sorghum leaf |
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| Authors: | D Tagu C Bergounioux C Cretin C Perennes P Gadal |
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| Institution: | (1) Laboratoire de Physiologie Végétale Moléculaire, U.A. CNRS 1128, Université de Paris-Sud, Centre d'Orsay, Orsay |
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| Abstract: | Summary Co-transformation experiments were carried out onPetunia hybrida protoplasts. The method used was electroporation with two plasmids: one confering kanamycin resistance, and the other harbouring a phosphoenolpyruvate carboxylase (PEPC) cDNA fromSorghum vulgare leaves. Southern blot analysis of the selected lines demonstrated a high co-transformation frequency.Abbreviations PEPC
phosphoenolpyruvate carboxylase
- NPT
neomycin phosphotransferase
- ATF
absolute transformation frequency
- PEG
polyethylenglycol
- BA
6-benzyl-aminopurine
- IAA
3-indoleacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid |
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| Keywords: | Phosphoenolpyruvate carboxylase Electroporation Sorghum Protoplasts |
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