Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum |
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Authors: | Li He-Ping Wu Ai-Bo Zhao Chun-Sen Scholten Olga Löffler Huub Liao Yu-Cai |
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Affiliation: | Molecular Biotechnology Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China. |
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Abstract: | Based on the intergenic sequences of Tri5-Tri6 genes involved in the mycotoxin pathways of Fusarium species, a generic PCR assay was developed to detect a 300 bp fragment of deoxynivalenol (DON)-chemotypes and a 360 bp sequence of nivalenol (NIV)- chemotypes of Fusarium graminearum. Mycotoxin chemotypes identified by the PCR assays were confirmed by the chemical analyses of HPLC or GC/MS. Further analysis of 364 F. graminearum isolates from 12 provinces of China showed that 310 were DON-chemotypes and 54 were NIV-chemotypes. Sequence analyses revealed that DON-chemotypes display more variations than NIV-chemotypes. This PCR assay could be used to detect mycotoxin-producing Fusarium-species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals. Also this assay may provide useful alternatives to antibody-based mycotoxin tests. |
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Keywords: | Fusarium graminearum Deoxynivalenol Nivalenol Polymerase chain reaction |
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