An improved micromethod for infectivity assays and neutralization tests of dengue viruses |
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| Authors: | T Ishimine M Tadano T Fukunaga Y Okuno |
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| Affiliation: | Department of Virology, School of Medicine, University of the Ryukyus, Okinawa, Japan. |
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| Abstract: | An improved micromethod for infectivity assays and neutralization (N) tests of dengue (DEN) type 1-4 viruses was developed, using 96-well plates and the PAP (peroxidase-antiperoxidase) staining technique. The foci formed on BHK-21 cell monolayers in wells of the plate were readily countable under an ordinary stereomicroscope. This micromethod has the advantages over the micromethod of the Lab-Tek 8 chamber slide system of lower cost, requirement for smaller volumes of test sera and applicability to larger number of serum specimens for N tests of DEN viruses. |
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