Differential effects of Cucumber mosaic virus satellite RNAs in the perturbation of microRNA-regulated gene expression in tomato |
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Authors: | Junli Feng Leiyu Lai Ruohong Lin Chunzhi Jin Jishuang Chen |
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Institution: | (1) Institute of Bioengineering, Zhejiang Sci-Tech University, Xiasha Road #2, Hangzhou, 310018, People’s Republic of China;(2) Institute of Bioresource Engineering, Nanjing University of Technology, Nanjing, China; |
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Abstract: | Viral infections generally cause disease symptoms by interfering with the microRNA (miRNA)-mediated regulation of gene expression
of host plants. In tomato leaves, the accumulation levels of eleven miRNAs and ten target mRNAs were enhanced by different
degrees upon Cucumber mosaic virus (CMV)-Fny and Tomato aspermy virus (TAV)-Bj infections. The ability of CMV-Fny to interfere with miRNA pathway was dramatically suppressed in the addition of
the benign satellite (sat) RNA variant (satYn12), but was slightly affected when CMV-Fny was co-inoculated with the aggressive satRNA variant (satT1). In plants harboring the infection of CMV-FnyΔ2b (a CMV-Fny 2b-deletion mutant), the unaltered miRNAs and target mRNAs
levels compared with mock inoculated plants indicated that 2b ORF was essential for perturbation of miRNA metabolism. When
the amounts of viral open reading frames (ORFs) in these infections were quantified, we found satYn12 caused a higher reduction of CMV-Fny accumulation levels than satT1. These results indicate the complex mechanism by which satRNAs participate in CMV-tomato interaction, and suggest that the severity of disease symptoms positively correlates to some
extent with the perturbation of miRNA pathway in tomato. |
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