Protein Profiling of the Dimorphic Pathogenic Fungus, Sporothrix schenckii |
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Authors: | Zhenying Zhang Binbin Hou Yi Xin Xiaoming Liu |
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Institution: | (1) Department of Dermatology and Venerology, 1st Affiliated Hospital of Dalian Medical University, Zhongshan Road 222, Dalian, Liaoning Province, China;(2) Proteomic Research Group, Department of Biological Sciences, Dalian Medical University, Dalian, Liaoning Province, China; |
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Abstract: | Sporotrichosis is a common cutaneous mycosis caused by the dimorphic fungus Sporothrix schenckii, which exhibits a temperature-dependent dimorphic switch. At 25°C, it grows in a mycelial phase, while at 37°C, it forms unicellular
yeast cells. The formation of yeast cells was thought to be a requisite for the pathogenicity of S. schenckii. To identify fragments that might be related to morphogenesis, whole-cell proteins from the mold and early yeast stages of
S. schenckii were analyzed using 2DE. Among thousands of protein molecules displayed, more than 300 showed a differential expression between
the two phases. In particular, 24 yeast-specific proteins were identified using MALDI-TOF/MS. One of the most interesting
proteins was a hybrid histidine kinase, DRK1, a global regulator of dimorphism and virulence in Blastomyces dermatitidis and Histoplasma capsulatum that was abundant in the yeast phase. Our study introduced a new approach to study dimorphism in S. schenckii, and the data may help us better understand the molecular mechanisms of phase transition. |
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