Construction and transformation of a Thermotoga-E. colishuttle vector |
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Authors: | Dongmei Han Stephen M Norris Zhaohui Xu |
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Affiliation: | (1) Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403, USA;(2) Center for Photochemical Sciences, Bowling Green State University, Bowling Green, OH 43403, USA |
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Abstract: | Background Thermotoga spp. are attractive candidates for producing biohydrogen, green chemicals, and thermostable enzymes. They may also serve as model systems for understanding life sustainability under hyperthermophilic conditions. A lack of genetic tools has hampered the investigation and application of these organisms. This study aims to develop a genetic transfer system for Thermotoga spp. |
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