| 动物转基因技术的新进展 |
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| 引用本文： | 李劲松,庄大中,孙青原,陈大元. 动物转基因技术的新进展[J]. 生物化学与生物物理进展, 2000, 27(2): 124-126 |
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| 作者姓名： | 李劲松 庄大中 孙青原 陈大元 |
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| 作者单位： | 中国科学院动物研究所,生殖生物学国家重点实验室,北京,100080 |
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| 基金项目： | 国家自然科学基金资助项目（39770098）. |
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| 摘 要： | 到目前为止，原核注射是最可靠，也是使用最广泛的动物转基因方法.但该方法存在整合效率太低及不能定点整合的问题.在过去的20年里，出现了一些新的转基因方法，包括精子介导、反转录病毒介导、携带外源基因体细胞的核移植、ＥＳ细胞基因打靶技术等.但这些方法都未能根本地解决存在的问题.最近的一些文献中报道转基因技术在原有方法的基础上做出了改进后，取得了突破性进展.
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| 关 键 词： | 转基因技术，显微注射，动物 |
| 收稿时间： | 1999-10-16 |
| 修稿时间： | 1999-10-16 |
New Advances in Animal Transgenic Technology |
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| LI Jin-Song,ZHUANG Da-Zhong,SUN Qing-Yuan and CHEN Da-Yuan. New Advances in Animal Transgenic Technology[J]. Progress In Biochemistry and Biophysics, 2000, 27(2): 124-126 |
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| Authors: | LI Jin-Song ZHUANG Da-Zhong SUN Qing-Yuan CHEN Da-Yuan |
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| Affiliation: | State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | The technique of pronuclear microinjcetion is the most reliable and popular method for transgenic livestock production to date. However, pronuclear microinjection has been proven to be inefficient in producing transgenics and in inserting a piece of DNA into a specific site in the host genome. In the past 20 years, some new approaches have been used. These include sperm-mediated DNA transfer, retroviral mediated DNA transfer into oocytes, somatic cell carrying exogenous genes nuclear transfer, the use of embryonic stem(ES) cell. But these ways can not solve existing problems thoroughly. In recent papers, the existing techniques have been improved and significant progress has been made in developing transgenic techniques. |
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| Keywords: | transgenic technique microinjection animal |
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