| 菠萝离体再生研究进展 |
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| 引用本文： | 孙婧朗,周慧晶,郑珂媛,戴锡玲,朱木兰. 菠萝离体再生研究进展[J]. 亚热带植物科学, 2022, 51(1): 73-80. DOI: 10.3969/j.issn.1009-7791.2022.01.012 |
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| 作者姓名： | 孙婧朗 周慧晶 郑珂媛 戴锡玲 朱木兰 |
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| 作者单位： | (1. 中国科学院分子植物科学卓越创新中心，上海 200032；2. 上海辰山植物园 / 中国科学院上海辰山植物科学研究中心 / 上海市资源植物功能基因组学重点实验室，上海 201602；3. 上海师范大学生命科学学院，上海 200234) |
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| 基金项目： | 国家重点研发计划项目(2019YFD1001105) |
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| 摘 要： | 菠萝(Ananas comosus)具有很高的食用、药用及观赏价值，但存在种子萌发率低、遗传转化困难、基因编辑体系缺乏等问题，其基础研究及优质种苗规模化繁育亟需高效、可靠的离体再生系统。本文对菠萝离体再生外植体选择、外植体消毒、基本培养基筛选、直接不定芽发生、间接不定芽发生、体细胞胚胎发生、不定根诱导及炼苗移栽等方面的研究进展进行综述，以期为菠萝细胞工程育种、遗传资源改良及种苗工厂化生产提供理论与技术参考。
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| 关 键 词： | 菠萝 离体再生 不定芽诱导 体胚发生 |
| 收稿时间： | 2021-10-08 |
Research Progress on in vitro Regeneration of Pineapple |
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| SUN Jing-lang,ZHOU Hui-jing,ZHENG Ke-yuan,DAI Xi-ling,ZHU Mu-lan. Research Progress on in vitro Regeneration of Pineapple[J]. Subtropical Plant Science, 2022, 51(1): 73-80. DOI: 10.3969/j.issn.1009-7791.2022.01.012 |
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| Authors: | SUN Jing-lang ZHOU Hui-jing ZHENG Ke-yuan DAI Xi-ling ZHU Mu-lan |
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| Affiliation: | (1. Chinese Academic Scientific Center for Excellence in Molecular Plant Science, Shanghai 200032, China; 2. Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center, Shanghai Chenshan Botanical Garden, Shanghai 201602, China; 3. College of Life Sciences, Shanghai Normal University, Shanghai 200234, China) |
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| Abstract: | Pineapple has high edible, medicinal and ornamental value, but there are problems such as low seed germination rate, difficulty in genetic transformation, and lack of gene editing system. Its basic research and large-scale breeding of high-quality seedlings urgently need efficient and reliable in vitro regeneration system. This paper reviewed the research progress in seven aspects: initial explant selection, explant sterilization, basic medium determination, direct adventitious bud formation, indirect adventitious bud formation, somatic embryogenesis, adventitious root induction, and seedling transplanting, in order to provide theoretical and technical reference for pineapple cell engineering breeding, genetic resource improvement and seedling factory production. |
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| Keywords: | Ananas comosus in vitro regeneration adventitious bud induction somatic embryogenesis |
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