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A new method for quantitative estimation of the virus particles number |
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| Authors: | A. S. Shebanova M. N. Savvateev N. V. Maluchenko D. Yu. Trofimov I. I. Agapov |
| Affiliation: | (1) School of Biology, Lomonosov Moscow State University, Leninskie gori 1, bld. 12, Moscow, 119992, Russia;(2) NT-MDT Co., State Research Institute of Physical Problems, Zelenograd, Moscow, Russia;(3) DNA-Technology Co., Moscow, Russia;(4) Research Institute of Transplantology and Artificial Organs, Moscow, Russia |
| Abstract: | The virus particles of live mumps virus vaccine widely used for vaccination in Russia have been detected and visualized by the atomic force microscopy. For quantitative estimation of the number of observed virus particles the special method has been developed. The presence of the vaccine virus protein component was tested by ELISA and dot-blot analysis. Using a quantitative real-time PCR assay the number of copies of viral RNA was estimated. The results of the quantitative estimation obtained by real-time PCR corresponded to the atomic force microscopy data. |
| Keywords: | atomic force microscopy live virus vaccine virus particles quantitative real-time PCR |
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