Nuclear translocation of the heterotrimeric CCAAT binding factor of <Emphasis Type="Italic">Aspergillus oryzae</Emphasis> is dependent on two redundant localising signals in a single subunit |
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Authors: | Hideya?Goda Takashi?Nagase Shinjiro?Tanoue Junya?Sugiyama Stefan?Steidl André?Tüncher Tetsuo?Kobayashi Norihiro?Tsukagoshi Axel?A?Brakhage Email author" target="_blank">Masashi?KatoEmail author |
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Institution: | (1) Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan;(2) Department of Molecular and Applied Microbiology, Leibniz-Institute for Natural Products Research and Infection Biology (HKI), Beutenbergstrasse 11a, D-07745 Jena, Germany;(3) Present address: MorphoSys AG, Lena-Christ-Str. 48, 82152 Martinsried, Germany;(4) Present address: The University of the Air, 101-2 Yagoto-honmachi, Nagoya 466-0825, Japan |
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Abstract: | The CCAAT-binding complex in the Aspergillus species, also known as the Hap complex, consists of at least three subunits, namely HapB, HapC and HapE. Each Hap subunit
contains an evolutionary conserved core domain. Recently, we have found that the HapC and HapE subunits do not carry a nuclear
localisation signal. Furthermore, when in complex with HapB, they are transported into the nucleus via a ‘piggy back mechanism’
in A. nidulans. To extend our findings to other filamentous fungi, we examined the nuclear localisation of the A. oryzae Hap subunits by analysing several GFP fusion proteins with these Hap subunits in the hap deletion strains of A. nidulans. The nuclear translocation of the A. oryzae complex was found to be dependent on two redundant localising signals in HapB. |
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Keywords: | Aspergillus oryzae Hap complex CCAAT-box Nuclear localisation Nuclear localisation signal |
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