| 东方田鼠四个种群线粒体D-loop区遗传多态性分析 |
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| 引用本文： | 谢建云,冯洁,邵伟娟,柏熊,胡建华,高诚. 东方田鼠四个种群线粒体D-loop区遗传多态性分析[J]. 兽类学报, 2007, 27(4): 407-412 |
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| 作者姓名： | 谢建云 冯洁 邵伟娟 柏熊 胡建华 高诚 |
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| 作者单位： | 上海实验动物研究中心,上海实验动物监督检验站,上海200032;上海计划生育科学研究所,上海,200032;上海实验动物研究中心,上海实验动物监督检验站,上海200032;上海西普尔-必凯实验动物有限公司,上海,200237 |
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| 摘 要： | 东方田鼠(Microtus fortis)主要分布在我国和俄罗斯、朝鲜、蒙古等地,我国是东方田鼠主要分布区,在东北、西北、黄河流域、长江流域和浙江、福建等省都有分布.
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| 关 键 词： | 东方田鼠 线粒体DNA(mt DNA) RFLP 遗传多态性 |
| 文章编号： | 1000-1050（2007）04-0407-06 |
| 收稿时间： | 2006-12-22 |
| 修稿时间： | 2007-08-10 |
Mitochondrial DNA D-loop polymorphism among four populations of Reed voles (Microtus fortis) |
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| XIE Jianyun,FENG Jie,SHAO Weijuan,BAI Xiong,HU Jianhua,GAO Cheng. Mitochondrial DNA D-loop polymorphism among four populations of Reed voles (Microtus fortis)[J]. Acta Theriologica Sinica, 2007, 27(4): 407-412 |
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| Authors: | XIE Jianyun FENG Jie SHAO Weijuan BAI Xiong HU Jianhua GAO Cheng |
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| Abstract: | Four populations of Reed voles (Microtus fortis) were used to check genetic polymorphisms of the mitochondrial DNA (mtDNA)D-loop by means of RFLP analysis.The PCR fragments of this region were digested with 15 restriction endonucleases: 10 with no variation and the other five including Eco T22I, Eco RV, Hap II, Hind III and Xba I, indicating polymorphisms among different individuals.Ten haplotypes were eventually recovered among sampled 40 individuals.Thegenetic divergence among these haplotypes ranged respectively from 0. 0014 to 0.0168. All vole samples clustered into two distinct branches:Voles from Dongting Lake and some voles from Qingtongxia comprised one branch,and voles from Heilongjiang and the rests from Qingtongxia comprised the other branch. These findings suggested that the mtDNA D-loop region in this species provides informative variations for population research and phylogeography in the future. |
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| Keywords: | Genetic polymorphism Mitochondrial DNA (mtDNA) RFLP Reed vole (Microtus fortis) |
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