Effect of fungal pellet morphology on enzyme activities involved in phthalate degradation |
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Authors: | Young-Mi Kim Hong-Gyu Song |
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Institution: | (1) Department of Microbiology, Faculty of Science, Kasetsart University, Jatujak, Bangkok, 10900, Thailand;(2) Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Jatujak, Bangkok, 10900, Thailand;(3) National Metal and Materials Technology Center, 114 Thailand Science Park, Pathumthani, 12120, Thailand |
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Abstract: | Pellet size of white rot fungus, Pleurotus ostreatus may affect the secretion of its degradative enzymes and accompanying biodegrading capability, but could be controlled by
several physical culture conditions in liquid culture. The pellet size of P. ostreatus was affected by the volume of inoculum, flask, and medium, but the agitation speed was the most important control factor.
At the lower agitation speed of 100 rpm, the large pellets were formed and the laccase activity was higher than that of small
pelleted culture at 150 rpm, which might be due to loose intrapellet structure. However, the biodegradation rates of benzylbutylphthalate
and dimethylphthalate were higher in the small pelleted culture, which indicated the involvement of other degradative enzyme
rather than laccase. The activity of esterase which catalyzes the nonphenolic compounds before the reaction of ligninolytic
enzymes was higher in the small pelleted culture, and coincided with the degradation pattern of phthalates. This study suggests
the optimization of pellet morphology and subsequent secretion of degradative enzymes is necessary for the efficient removal
of recalcitrants by white rot fungi. |
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