Cytokinin-Mediated Axillary Shoot Formation in Pinus heldreichii

Shoot formation from seedling explants of Pinus heldreichii was induced by pulse treatment with benzyladenine at different concentration, followed by culture on medium lacking plant growth regulators. After treatment with 222 M benzyladenine (BA) an average of 4.6 shoots per explant was obtained. Shoots, detached from explants, rooted with a frequency of about 10 %, and rooted plantlets were successfully transferred to ex vitro conditions.