用PCR方法证实HIV－1感染性病毒颗粒携带有前病毒DNA

用多聚酶链反应（ＰＣＲ）为检测手段，证明了成熟的人免疫缺陷病毒１型（ＨＩＶ－１）可携带前病毒ＤＮＡ；用细胞病毒裂解液处理病毒样品或在病毒样品中只加磷酸盐缓冲液（ＰＢＳ）代替细胞裂解液，结果发现只有病毒裂解液处理样品后才能检测到特异ＤＮＡ．完整的病毒颗粒对ＤＮＡ酶不敏感，而只有在病毒裂解以后才能对ＤＮＡ酶敏感。当靶细胞膜上的ＣＤ＿４受体被特异的单克隆抗体覆盖后不能吸附病毒时，ＤＮＡ也不能被检测到，而且ＤＮＡ的数员和病毒滴度的高低相一致。这些证据都提示这种和病毒相关的ＤＮＡ存在于完整的有感染性的成熟病毒颗粒内部。

Demonstration of Infectious Mature HIV-1 Carrying Pre-viral DNA by PCR

nfectious mature human immunodeficiency virus type 1 (HIV-1) carruig pre-virus DNA was evidenced by pelpoerase chani reaction(PCR).When the samples were treated with lytic buffer,the result of PCR should be positive;however when PBS was added uistead of lytic buffer,the DNA wasnot detected. The DNA was sensitive to DNase treamient after lysisof viral particles.However,theDNA was not seasitive to DNase treatinent for the intact viral paricles.The DNA was not detected after the CD_4 receptor of the C8_(166) cells were covered by anti-CD_4 receptor monoclonal antibody(Leu3a),and the virus could not absorb to the cells.Furthermore,the number of DNA was correlativewith virus titer.This evidence supported the HIV-I virus carrying pre-virus DNA.