An Integrated Map with Cosmid/PAC Contigs of a 4-Mb Down Syndrome Critical Region |
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| Authors: | Kazutoyo Osoegawa Rie Susukida Saishi Okano Jun Kudoh Shinsei Minoshima Nobuyoshi Shimizu Pieter J de Jong Juergen Groet Jane Ives Hans Lehrach Dean Nizetic Eiichi Soeda |
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| Institution: | aRIKEN Gene Bank, The Institute of Physical and Chemical Research, 3-1-1 Koyadai, Tsukuba, 305, Japan;bDepartment of Molecular Biology, Keio University School of Medicine, Shinjuku-ku, Tokyo, 160, Japan;cRoswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, New York, 14263-0001;dCentre for Applied Molecular Biology, The School of Pharmacy, University of London, 29/39 Brunswick Square, London, WC1N 1AX, United Kingdom;eImperial Cancer Research Fund, P.O. Box 123, Lincoln's Inn Fields, London, WC 2A 3 PX, United Kingdom |
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| Abstract: | The major phenotypic features of Down syndrome have been correlated with partial trisomies of chromosome 21, allowing us to define the candidate gene region to a 4-Mb segment on the 21q22.2 band. We present here a high-resolution physical map with megabase-sized cosmid/PAC contigs. This ordered clone library has provided unique material for the integration of a variety of mappable objects, including exons, cDNAs, restriction sites, etc. Furthermore, our results have exemplified a strategy for the completion of the chromosome 21 map to sequencing. |
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