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| 极端耐热木聚糖酶基因在枯草芽孢杆菌中的整合表达 | |
| 引用本文： | 张伟,李冠,娄恺.极端耐热木聚糖酶基因在枯草芽孢杆菌中的整合表达[J].生物技术,2010,20(1):15-18. |
| 作者姓名： | 张伟 李冠 娄恺 |
| 作者单位： | 1. 新疆农科院微生物研究所;新疆大学生命科学与技术学院,新疆,乌鲁木齐,830046 2. 新疆大学生命科学与技术学院,新疆,乌鲁木齐,830046 3. 新疆农科院微生物研究所 |
| 基金项目： | 新疆自治区高新技术项目 |
| 摘 要： | 目的:为了在枯草芽孢杆菌中整合表达极端耐热木聚糖酶。方法:将嗜热网球菌(Dictyoglomus thermophilum)Rt46B.1的极端耐热木聚糖酶基因xynB通过穿梭载体pDL整合到B.subtilis168染色体上,使其实现表达。结果:极端耐热木聚糖基因在枯草芽孢杆菌中成功整合并表达。结论:基因工程菌B.subtilis168-xynB能外泌表达极端耐热木聚糖酶,且表达水平为0.732IU/mL,比在大肠杆菌中的高。酶学性质表明,此酶分子量约为24kD,其最适反应温度为85℃,最适反应pH值为6.5,且在弱碱性条件下稳定。 |
| 关 键 词： | 极端耐热木聚糖酶基因 枯草芽孢杆菌 同源重组 双交换整合 |
Expression of an Extreme-thermostable Xylanase B from Dictyoglomus thermophilum in B.subtilis168 |
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| ZHANG Wei,LI Guan,LOU Kai.Expression of an Extreme-thermostable Xylanase B from Dictyoglomus thermophilum in B.subtilis168[J].Biotechnology,2010,20(1):15-18. | |
| Authors: | ZHANG Wei LI Guan LOU Kai |
| Institution: | ZHANG Wei1,2,LI Guan2,LOU Kai1(1.Institute of Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830000,China,2.Colloge of Life Science , Technology,Xinjiang University,Urumqi 830046,China) |
| Abstract: | Objective: In order to expressive the extreme thermophile xylanase gene xynB from Rt46B.1(Dictyoglomus thermophilum).Method: The gene was cloned into the integration vectors of pDL and transform into host cell of B.subtilis 168.Result:The gene of xynB was recombinanted into chromatic body of B.subtilis 168 by double crossover homologous sequence substitution.Conclusion: The recombinant which has chlorampenicol resistance can express xylanase activity.Xylanase B(24kD) was successfully expressed(0.732IU/mL) w... |
| Keywords: | thermostable xylanase gene B subtilis168 homologous recombination double crossover integration |
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