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A simple colorimetric method for testing antimicrobial susceptibility of biofilmed bacteria |
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| Authors: | Kim Shukho Kim Mi Jin Kang Hee Young Seol Sung Yong Cho Dong Taek Kim Jungmin |
| Affiliation: | (1) Department of Clinical Microbiology, Penteli Children’s Hospital, Athens, Greece;(2) Department of Microbiology, Medical School, University of Athens, Athens, Greece;(3) Department of Statistics and Insurance Science, University of Piraeus, Piraeus, Greece;(4) Laboratory of Biopathology and Clinical Microbiology, Aeginition Hospital, Medical School, University of Athens, Vass. Sophias av. 72–74, 115 28 Athens, Greece;(5) Department of Nursing, Faculty of Human Movement and Quality of Life Sciences, University of Peloponnese, Peloponnese, Greece; |
| Abstract: | This study introduces a simple colorimetric method which can measure the antimicrobial susceptibility of bacteria in biofilms using trimethyl tetrazolium chloride (TTC) as an indicator of viable bacteria. The new method was utilized for the evaluation of antibiotic susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus biofilms. |
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