Effect of 0.25 N sodium chloride treatment on DNA denaturation in situ in thymus lymphocytes |
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| Authors: | Z Darżynkiewicz F Traganos T Sharpless MR Melamed |
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| Institution: | Sloan-Kettering Memorial Cancer Center, New York, NY 10021, USA |
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| Abstract: | Extraction of cells with 0.25 N NaCl changes the profile of DNA denaturation in situ. The portion of DNA denaturing at lower temperatures (“thermosensitive” fraction) shows increased sensitivity to heat following salt extraction while the “thermoresistant” DNA fraction is further stabilized. The results suggest that proteins extractable with 0.25 N NaCl while providing local counterions for DNA phosphates of the “thermosensitive” DNA fraction also decrease the strength of DNA-histone interactions within the “thermoresistant” fraction. |
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