Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy |
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Authors: | Stuart J Corr Brandon T Cisneros Leila Green Mustafa Raoof Steven A Curley |
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Institution: | 1.Department of Surgical Oncology, University of Texas M.D. Anderson Cancer Center;2.Department of Chemistry, Rice University;3.Mechanical Engineering and Materials Science, Rice University |
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Abstract: | Cancer therapies which are less toxic and invasive than their existing counterparts are highly desirable. The use of RF electric-fields that penetrate deep into the body, causing minimal toxicity, are currently being studied as a viable means of non-invasive cancer therapy. It is envisioned that the interactions of RF energy with internalized nanoparticles (NPs) can liberate heat which can then cause overheating (hyperthermia) of the cell, ultimately ending in cell necrosis.In the case of non-biological systems, we present detailed protocols relating to quantifying the heat liberated by highly-concentrated NP colloids. For biological systems, in the case of in vitro experiments, we describe the techniques and conditions which must be adhered to in order to effectively expose cancer cells to RF energy without bulk media heating artifacts significantly obscuring the data. Finally, we give a detailed methodology for in vivo mouse models with ectopic hepatic cancer tumors. |
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Keywords: | Medicine Issue 78 Electronics and Electrical Engineering Life Sciences (General) Radiofrequency Cancer Nanoparticles Hyperthermia Gold |
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