Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis |
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Authors: | Syed M.Q. Hussaini Heechul Jun Chang Hoon Cho Hyo Jin Kim Woon Ryoung Kim Mi-Hyeon Jang |
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Affiliation: | 1.Department of Neurologic Surgery, Mayo Clinic College of Medicine;2.Department of Anatomy, Korea University College of Medicine;3.Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine |
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Abstract: | Traditional methods of immunohistochemistry (IHC) following tissue fixation allow visualization of various cell types. These typically proceed with the application of antibodies to bind antigens and identify cells with characteristics that are a function of the inherent biology and development. Adult hippocampal neurogenesis is a sequential process wherein a quiescent neural stem cell can become activated and proceed through stages of proliferation, differentiation, maturation and functional integration. Each phase is distinct with a characteristic morphology and upregulation of genes. Identification of these phases is important to understand the regulatory mechanisms at play and any alterations in this process that underlie the pathophysiology of debilitating disorders. Our heat-induced antigen retrieval approach improves the intensity of the signal that is detected and allows correct identification of the progenitor cell type. As discussed in this paper, it especially allows us to circumvent current problems in detection of certain progenitor cell types. |
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Keywords: | Neuroscience Issue 78 Neuroscience Neurodegenerative Diseases Nervous System Diseases Behavior and Behavior Mechanisms adult neurogenesis hippocampus antigen retrieval immunohistochemistry neural stem cell neural progenitor |
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