Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia |
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Authors: | Tristan M. Nicholson Kristen S. Uchtmann Conrad D. Valdez Ashleigh B. Theberge Tihomir Miralem William A. Ricke |
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Affiliation: | 1.Department of Urology, University of Wisconsin-Madison;2.Medical Scientist (MD/PhD) Training Program, University of Rochester School of Medicine & Dentistry;3.Molecular and Environmental Toxicology Center, University of Wisconsin-Madison |
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Abstract: | New therapies for two common prostate diseases, prostate cancer (PrCa) and benign prostatic hyperplasia (BPH), depend critically on experiments evaluating their hormonal regulation. Sex steroid hormones (notably androgens and estrogens) are important in PrCa and BPH; we probe their respective roles in inducing prostate growth and carcinogenesis in mice with experiments using compressed hormone pellets. Hormone and/or drug pellets are easily manufactured with a pellet press, and surgically implanted into the subcutaneous tissue of the male mouse host. We also describe a protocol for the evaluation of hormonal carcinogenesis by combining subcutaneous hormone pellet implantation with xenografting of prostate cell recombinants under the renal capsule of immunocompromised mice. Moreover, subcutaneous hormone pellet implantation, in combination with renal capsule xenografting of BPH tissue, is useful to better understand hormonal regulation of benign prostate growth, and to test new therapies targeting sex steroid hormone pathways. |
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Keywords: | Medicine Issue 78 Cancer Biology Prostatic Hyperplasia Prostatic Neoplasms Neoplastic Processes Estradiol Testosterone Transplantation Heterologous Growth Xenotransplantation Heterologous Transplantation Hormones Prostate Testosterone 17beta-Estradiol Benign prostatic hyperplasia Prostate Cancer animal model |
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