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Cloning and Characterization of HumanSep1 (hSEP1) Gene and Cytoplasmic Localization of its Product |
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| Authors: | Sato Yoshiki; Shimamoto Akira; Shobuike Takeo; Sugimoto Masanobu; Ikeda Hideo; Kuroda Shigetoshi; Furuichi Yasuhiro |
| Institution: | 1AGENE Research Institute 200 Kajiwara, Kamakura, Kanagawa, 247-0063 2Department of Neuropsychiatry, Okayama University Medical School 2-5-1 Shikata-cho, Okayama 700-0914 3Department of Molecular Biology, Institute of Medical Science, University of Tokyo P.O. Takanawa, Tokyo 108-0074, Japan |
| Abstract: | We isolatedand sequenced a human cDNA (designated as hSEP1)encoding both a homologue of mouse Dhm2 and budding yeast SEP1.The gene was shown to be locatedon the long arm of chromosome3 (3q25-26.1). The putative hSEP1 product (hSEP1p) consistedof 1694 amino acid residues with a molecular mass of about 190kDa. Northern blot analysis showeda major 10-kb mRNA expressedubiquitously in variousorgans as well as a minor 5.5-kb mRNAexpressed relatively highly in the testis and placenta. hSEP1pis localizedin the cytoplasm as examinedb y cytochemical andWestern blot analyses of fractionated cellular extracts. Thebiological function of hSEP1p was discussed in correlation withits cytoplasmic localization. |
| Keywords: | hSEP1 Dhm2 SEP1 human cell |
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