High anti-Helicobacter pylori antibody seropositivity associated with the combination of IL-8-251TT and IL-10-819TT genotypes |
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Authors: | Hamajima Nobuyuki Katsuda Nobuyuki Matsuo Keitaro Saito Toshiko Hirose Kaoru Inoue Manami Zaki Toshiro Take Tajima Kazuo Tominaga Suketami |
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Institution: | Division of Epidemiology and Prevention, Aichi Cancer Center Research Center, Nagoya, Japan;;;Aichi Cancer Center, Nagoya, Japan |
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Abstract: | Background. Helicobacter pylori induces inflammation of gastric mucosa regulated by several interleukins. This study examined associations between anti‐Helicobacter pylori immunoglobulin G antibody seropositivity and functional polymorphisms of interleukin‐8 T‐251 A and interleukin‐10 T‐819C. Materials and Methods. The subjects were 454 health check‐up examinees (126 males and 328 females) without a history of cancer, aged 35–85 years, residing in Nagoya, Japan. After written informed consent was obtained individually, residual blood was anonymously applied for anti‐Helicobacter pylori immunoglobulin G antibody testing and genotyping by the polymerase chain reaction with confronting two‐pair primers. Results. The genotype frequency of interleukin‐8 T‐251 A was 52.2% for TT, 39.5% for TA, and 8.3% for AA, and that of interleukin‐10 T‐819C was 49.5% for TT, 39.9% for TC and 10.6% for CC. Although the differences in the positive rates among the genotypes were not marked, 115 individuals with interleukin‐8–251TT (low expression genotype) and interleukin‐10–819TT (high expression genotype) had a higher rate (63.5%) than the others (52.0%). Relative to the combination of interleukin‐8–251TT and interleukin‐10–819TT, the sex‐age‐adjusted odds ratio for those with the other combinations was 0.62 (95% confidence interval, 0.39–0.98). The adjusted odds ratio among 65 current smokers was 0.13 (0.03–0.61). Conclusions. The observed association suggests that individuals with interleukin‐8–251TT and interleukin‐10–819TT, a combination presumably causing mild inflammation, have a higher probability of the continuing Helicobacter pylori infection, especially among current smokers. |
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Keywords: | Helicobacter pylori seropositivity interleukin-8 T-251A interleukin-10 T-819C polymerase chain reaction with confronting two-pair primers |
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