High malic enzyme activity in tumor cells and its cross-reaction with antipigeon liver malic enzyme serum |
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Authors: | Pui-ling Kam Ching-chun Lin Jue-Cheng Li Ching-liang Meng Gu-gang Chang |
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Institution: | (1) Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, Republic of China;(2) Department of Dentistry, National Defense Medical Center, Taipei, Taiwan, Republic of China |
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Abstract: | Summary Rabbit antibodies against pigeon liver malic enzyme (EC 1.1.1.40) were prepared. The antiserum gave single precipitation line
with crude pigeon liver extract. Cross reaction was observed with partially purified malic enzyme or crude extract from chicken
liver. Positive cross reaction was also observed with the concentrated cytosolic fraction of two human carcinoma cell lines
which were demonstrated to contain high malic enzyme activity. All other proteins examined did not react with the antibodies.
When purified pigeon liver malic enzyme was mixed with the antiserumin vitro, a time-dependent inactivation of the enzyme activity was observed. Protection of the enzyme activity against antiserum inactivation
was afforded by NADP+ orL-malate. Metal Mn2+ gave little protection. |
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Keywords: | (tumor pigeon liver) malic enzyme polyclonal antibodies inactivation cross-reaction specificity |
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