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| 猪胚胎开放式拉长细管玻璃化冷冻保存研究 | |
| 引用本文： | 张德福 刘东 吴华丽 郑筱峰 王昭凯 王少兵. 猪胚胎开放式拉长细管玻璃化冷冻保存研究[J]. 生物工程学报, 2006, 22(5): 845-849 |
| 作者姓名： | 张德福 刘东 吴华丽 郑筱峰 王昭凯 王少兵 |
| 作者单位： | 1. 上海农业科学院畜牧兽医研究所,上海,201106;上海市农业遗传育种重点实验室动物遗传工程研究室,上海,201106 2. 南京农业大学动物医学院,南京,210095 3. 南京农业大学动物科技学院,南京,210095 |
| 基金项目： | 上海市科技兴农科技攻关项目 |
| 摘 要： | 从20头供体母猪获得的291枚可用胚胎(囊胚/桑葚胚),采用二步法开放式拉长细管(OPS,openpulledstraw)玻璃化冷冻技术进行保存,即胚胎首先在冷冻液I(TCM199 20?S 10%EG 10%DMSO)中平衡3min,然后立即转入冷冻液II(TCM199 20?S 20%EG 20%DMSO 0.4mol/LSUC)中并在1min内装管,直接投入液氮保存;3个月后解冻移植给8头受体母猪,其中1头怀孕产仔(8头活仔),在我国首次获得猪胚胎超低温(-196℃)冷冻后代。 |
| 关 键 词： | 猪 冷冻胚胎 玻璃化冷冻 |
| 文章编号： | 1000-3061（2006）05-0845-05 |
| 收稿时间： | 2006-03-28 |
| 修稿时间： | 2006-05-31 |
Study on Vitrification of Porcine Embryos by Open Pulled Straw Method |
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| ZHANG De-Fu,LIU Dong,WU Hua-Li,ZHENG Xiao-Feng,WANG Zhao-Kai,WANG Shao-Bing. Study on Vitrification of Porcine Embryos by Open Pulled Straw Method[J]. Chinese journal of biotechnology, 2006, 22(5): 845-849 | |
| Authors: | ZHANG De-Fu LIU Dong WU Hua-Li ZHENG Xiao-Feng WANG Zhao-Kai WANG Shao-Bing |
| Affiliation: | Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, China. zhangdefu10@yahoo.com.cn |
| Abstract: | 291 embryos (Blastocyst/Morula) from 20 donor sows were vitrified by two step method with OPS (open pulled straw) in solution I (TCM199 + 20% FBS + 10% EG + 10% DMSO) for 3min, and solution II (TCM199 + 20% FBS + 20% EG + 20% DMSO + 0.4mol/L SUC) for 1min, stored in liquid nitrogen for 3 months, and transferred into 8 recipient sows after warming, one recipient sow was pregnant and 8 alive piglets were born. This is the first paper to report getting alive piglets by vitrification in China. |
| Keywords: | pig frozen embryos vitrification |
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