|
|||||
|
|
Isolation and sequence of a tomato cDNA clone encoding subunit II of the photosystem I reaction center |
|
| Authors: | Neil E. Hoffman Eran Pichersky Vedpal S. Malik Kenton Ko Anthony R. Cashmore |
| Affiliation: | (1) Plant Science Institute, Department of Biology, University of Pennsylvania, 19104 Philadelphia, PA, USA;(2) Present address: Department of Plant Biology, Carnegie Institute of Washington, 290 Panama Street, 94305 Stanford, CA, USA;(3) Department of Biology, University of Michigan, 48109 Ann Arbor, MI, USA;(4) Philip Morris Research Center, P.O. Box 26583, 23261 Richmond, VA, USA |
| Abstract: | We report here the isolation and nucleotide sequence of a cDNA clone encoding a phtosystem I polypeptide that is recognized by a polyclonal antibody prepared against subunit II of the photosystem I reaction center. The transit peptide processing site was determined to occur after Met50 by N terminal sequencing. The decuced sequence of this protein predicts that the polypeptide has a net positive charge (pI=9.6) and no membrane spanning regions are evident from the hydropathy plot. Based on these considerations and the fact that subunit II is solubilized by alkali treatment of thylakoids, we concluded that subunit II is an extrinsic membrane protein. The absence of hydrophobic regions characteristic of thylakoid transfer domains furthermore implies that subunit II is localized on the stromal side of the membrane. |
| Keywords: | electron transfer light-harvesting complex I membrane localization photosynthesis processing site transit peptide |
| 本文献已被 SpringerLink 等数据库收录！ | |