Strain-specific antisera to identify Thai Bradyrhizobium japonicum strains in preserved soybean nodules |
| |
Authors: | Payakapong Waraporn Tittabutr Panlada Boonkerd Nantakorn |
| |
Institution: | (1) School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand |
| |
Abstract: | Strain-specific antisera were produced against six Bradyrhizobium japonicum strains using two immunization procedures. These specific antisera were used for detection of bradyrhizobia in preserved soybean nodules. Antisera specific for two of these strains were either conjugated with a fluorescent dye or used with a fluorescent secondary antibody for identification of bradyrhizobia in soybean nodules that were preserved in four different storage conditions. Results show that soybean nodules dried in the oven, stored under room temperature, or at –20 °C are as suitable as fresh nodules for strain identification using fluorescent antisera. |
| |
Keywords: | Antisera production Bradyrhizobium soybean nodules |
本文献已被 SpringerLink 等数据库收录! |