A convenient assay for detection of soluble interferon receptors |
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| Authors: | S Stefanos S Pestka |
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| Affiliation: | Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, Piscataway. |
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| Abstract: | The most common problem that must be overcome in purification of plasma membrane receptors from a cell line or a tissue is the loss of activity after solubilization with a detergent. This paper describes a simple dot blot assay with 32P-labeled human interferon gamma (Hu-IFN-gamma) for the detection of a functionally active Hu-IFN-gamma receptor from soluble plasma membranes prepared from human placenta. |
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