Response of electrically stimulated cells of Pseudomonas oleovorans strain ATCC 29347 suspended in silicone oil |
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Authors: | Anglade J Hirschler A Le Petit J Matheron R Scarpitta A Iacazio G |
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Institution: | Laboratoire de Microbiologie, IMEP, CNRS UPRES A 6116, Faculté des Sciences et Techniques de Saint-Jérome, Marseille, France. m8700576@educ005-u.3mrs.fr |
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Abstract: | A high intensity direct current was applied for more than 10 min onto a bacterial suspension of Pseudomonas oleovorans ATCC 29347 suspended in silicone oil. The application of a gradually increased electric field from 0 to 2500 V x cm(-1) resulted in a decrease of the optical density of the bacterial suspension and the occurrence of a peak current of several hundred microA for living cells instead of a linear increase (few microA) for killed or lyophilised cells. This procedure is not only a rapid way of investigating the living state of cell cultures but also an efficient experimental tool to study the cellular effects of a controlled electrical stress. |
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Keywords: | DC current Gradual increase Silicone oil Physiological state Peak current Pseudomonas oleovorans |
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