Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS |
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Authors: | Lembcke Jan Ceglarek Uta Fiedler Georg Martin Baumann Sven Leichtle Alexander Thiery Joachim |
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Affiliation: | Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, Germany. |
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Abstract: | A novel analytical platform based on liquid chromatography and tandem mass spectrometry using atmospheric pressure photoionization was applied for the simultaneous quantification of free and esterified beta-sitosterol, campesterol, brassicasterol, and stigmasterol. The total time for sample pretreatment and analysis could be reduced from approximately 3 h [gas chromatography-mass spectrometry (GC-MS)] to 15 min. The detection limits of the different phytosterols ranged between 0.25 and 0.68 microg/l. Linear ranges were between 1 and 1,000 microg/l. The within-run and between-run variabilities ranged between 1.4% and 9.9%. The analytical sensitivity was at least 150-fold higher compared with GC-MS. Our new method allows a rapid and simultaneous determination of free and esterified phytosterols in serum. |
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Keywords: | free phytosterols phytosterol esters liquid chromatography plant sterols |
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