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Combining induced mutation and protoplasting for strain improvement of Aspergillus oryzae for kojic acid production |
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| Authors: | Hisao-Ming Wan Chien-Chiang Chen Te-Sheng Chang Raghavan N. Giridhar Wen-Teng Wu |
| Affiliation: | Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30043, Taiwan. |
| Abstract: | By combining induced mutation, using NTG and UV irradiation, and protoplasting of a wild type strain of Aspergillus oryzae ATCC 22788, a hyper-producing strain was obtained that accumulated 41 g kojic acid l(-1) in shake-flasks, which was 100-fold higher than that in the wild type strains. Similar production of kojic acid was obtained in 5 l stirred-tank fermentations. |
| Keywords: | Aspergillus oryzae kojic acid protoplasting strain improvement UV irradiation |
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