Correlation of changes in cardiac calcium channels with hemodynamics in Syrian hamster cardiomyopathy and heart failure |
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| Authors: | M S Finkel E S Marks R E Patterson E H Speir K A Steadman H R Keiser |
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| Affiliation: | 1. Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung, 804201, Taiwan;2. Division of Cardiology, Department of Internal Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung, 80284, Taiwan;3. Institute of BioPharmaceutical Sciences, National Sun Yat-Sen University, Kaohsiung, 804201, Taiwan;4. Division of Cardiovascular Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;5. Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan;6. Department and Graduate Institute of Pharmacology, National Defense Medical Center, Taipei, Taiwan;1. School of Medicine, China Medical University, Taichung, 40402, Taiwan;2. Division of Nephrology, Department of Internal Medicine, China Medical University Hospital, Taichung, 40402, Taiwan;3. Clinical Nutrition, China Medical University Hospital, Taichung, 40402, Taiwan;4. Department of Emergency, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, 427, Taiwan;5. Division of Neurosurgery, Department of Surgery, College of Medicine and Hospital, National Taiwan University, Taipei 10051, Taiwan;6. Department of Nutrition, China Medical University, Taichung, Taiwan, 40402, ROC;7. School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan;8. Department of Nursing, College of Medical and Health Science, Asia University, Taichung, 413, Taiwan;9. Institute of Nuclear Energy Research, Atomic Energy Council, Taoyuan, 32546, Taiwan;10. Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, 10051, Taiwan;11. Institute of Medicine, Chung Shan Medical University, Taichung, 40201, Taiwan;12. Department of Medical Research, Chung Shan Medical University Hospital, Taichung, 40201, Taiwan;13. Master Program of Food and Drug Safety, China Medical University, Taichung, 406040, Taiwan;1. Department of Anesthesiology, Pharmacology & Therapeutics, D Department of Chemistry, University of British Columbia (UBC), Vancouver, Canada;2. Centre for Heart Lung Innovation, University of British Columbia (UBC), Vancouver, Canada;3. Research Center of the Centre Hospitalier Universitaire de Sherbrooke, Québec, Canada;4. Department of Medicine, Endocrinology Division, Université de Sherbrooke, Québec, Canada |
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| Abstract: | We compared hemodynamics with [3H]nitrendipine (calcium channel) binding to cardiac membranes from Bio 14.6 cardiomyopathic Syrian hamsters at 4 and 10 months with their F1B controls. A 50% increase in the number (Bmax) of nitrendipine binding sites (calcium channels) was seen only in the 4 month old myopathic vs controls (Bmax = 468 +/- 11 vs 309 +/- 10 fmol/mg prot with no change in affinity (KD) (KD = .65 +/- .12 vs .75 +/- .14 nM), while no differences in Bmax or KD were seen at 10 months (Bmax = 375 +/- 9 vs 362 +/- 7 fmol/mg prot/KD = .82 +/- .18 vs .89 +/- .17 nM) myopathic vs control respectively. Hemodynamic studies revealed no significant differences in cardiac output, cardiac index, stroke volume, heart rate, mean arterial pressure, peripheral resistance, body weight, heart weight at 4 months, but a significant decrease in peripheral resistance (1120 +/- 360 vs 2080 +/- 240) increase in body weight (118 +/- 2 vs 94 +/- 2 grams) and heart weight (97 +/- 5 vs 78 +/- 2 gms/100 gms body weight) in 10 month myopathic vs control animals. We conclude that the onset of cardiomyopathy at 4 months is associated with a selective increase in calcium channel binding sites and heart failure at 10 months is associated with a relative decrease in these sites. |
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