Multiple pathways regulate endocytic coat disassembly in Saccharomyces cerevisiae for optimal downstream trafficking |
| |
Authors: | Toret Christopher P Lee Linda Sekiya-Kawasaki Mariko Drubin David G |
| |
Institution: | Department of Molecular and Cell Biology, University of California, 16 Barker Hall, Berkeley, CA 94720-3202, USA; Current address: Center for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, BC, Canada V5Z 4H4; Current address: Molecular Biology Laboratory, RIKEN Discovery Research Institute, Hirosawa, Wako Saitama 351-0198, Japan |
| |
Abstract: | Recently, a pathway involving the highly choreographed recruitment of endocytic proteins to sites of clathrin/actin-mediated endocytosis has been revealed in budding yeast. Here, we investigated possible roles for candidate disassembly factors in regulation of the dynamics of the endocytic coat proteins Sla2p, Ent1p, Ent2p, Sla1p, Pan1p and End3p, each of which has mammalian homologues. Live cell imaging analysis revealed that in addition to the synaptojanin, Sjl2p, the Ark1p and Prk1p protein kinases, the putative Arf GTPase-activating protein, Gts1p and the Arf GTPase-interacting protein, Lsb5p, also arrive at endocytic sites late in the internalization pathway, consistent with roles in coat disassembly. Analysis of coat dynamics in various mutant backgrounds revealed that multiple pathways, including the ones mediated by an Arf guanosine triphosphatase and a synaptojanin, facilitate efficient disassembly of different endocytic coat proteins. In total, at least four separate processes are important for disassembly of endocytic complexes and efficient downstream trafficking of endocytic cargo. |
| |
Keywords: | Arf GTPase auxilin endocytosis epsin synaptojanin uncoating |
本文献已被 PubMed 等数据库收录! |
|