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Isolation of mitochondria from Plasmodium falciparum showing dihydroorotate dependent respiration. |
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| Authors: | Eizo Takashima Shinzaburo Takamiya Satoru Takeo Fumika Mi-ichi Hisako Amino and Kiyoshi Kita |
| Institution: | a Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan b Department of Parasitology, School of Medicine, Juntendo University, Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan |
| Abstract: | Using N2 cavitation, we established a protocol to prepare the active mitochondria from Plasmodium falciparum showing a higher succinate dehydrogenase activity than previously reported and a dihydroorotate-dependent respiration. The fact that fumarate partially inhibited the dihydroorotate dependent respiration suggests that complex II (succinate–ubiquinone reductase/quinol–fumarate reductase) in the erythrocytic stage cells of P. falciparum functions as a quinol–fumarate reductase. |
| Keywords: | Plasmodium falciparum Mitochondria Dihydroorotate oxidase Fumarate reductase Succinate dehydrogenase |
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