Conversion of crude chitosan to an anti-fungal protease by <Emphasis Type="Italic">Bacillus cereus</Emphasis> |
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Authors: | Wen-Teish Chang Cheng-Hong Hsieh Hung-Sheng Hsieh Chinshuh Chen |
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Institution: | (1) Department of Food Science and Biotechnology, National ChungHsing University, 250, Kuokuang Rd., Taichung, Taiwan, ROC;(2) Department of Health and Nutrition Biotechnology, Asia University, Lioufeng, Wufeng 500, Lioufeng Rd., Taichung County, Taiwan, ROC |
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Abstract: | Bacillus cereus AU004, isolated from soil samples, secreted a complex of hydrolytic enzymes into the culture broth when it was grown aerobically
in a medium containing crude chitosan flakes. The presence of the AU004 culture supernatant substantially influenced the growths
of the plant-pathogenic fungi Fusarium oxysporum, F. solani and Pythium ultimum in terms of dry weight. AU004 excreted a protease when cultivated in a medium that contained 4% (w/v) chitosan as the major
nutritional source. The protease was purified by sequential chromatography and characterized as a novel extracellularly neutral
protease. The protease had an Mr of 28.8 kDa. The optimal pH and temperature for protease activity were 7 and 50°C, respectively.
Antifungal activity of the protease was observed using an assay based on the inhibition of spore germination and hyphal extension
of the fungal Pythium ultimum. This investigation is the first report of the production of an anti-fungal protease from Bacillus spp. |
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Keywords: | Antifungal Bacillus cereus Chitosan Protease Pythium ultimum |
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