Detection of microsatellite instability during somatic embryogenesis of oak (Quercus robur L.) |
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| Authors: | E Wilhelm K Hristoforoglu S Fluch K Burg |
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| Institution: | (1) Division of Environment and Life Sciences, Department of Biotechnology, ARC Seibersdorf Research, 2444 Seibersdorf, Austria;(2) Horticultural College and Research Institutes, Grünbergstrasse 24, 1131 Vienna, Austria |
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| Abstract: | Five microsatellite loci (QpZAG1/5, QpZAG9, QpZAG36, MSQ4, MSQ13) were used to test for genetic stability of three somatic embryogenic culture lines of Quercus robur L. and plantlets derived therefrom. DNA variation was detected among somatic embryos within all embryogenic lines, whereas no genetic instability was found among the regenerated plants. Two microsatellite loci revealed variation, and a locus-dependent instability was observed. The most polymorphic and useful microsatellite locus for detecting genetic variation was QpZAG9, with 28.5% of the investigated loci being variable. |
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| Keywords: | Simple sequence repeats Somaclonal variation Somatic embryogenesis In vitro culture Quercus ssp |
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