Increasing <Emphasis Type="Italic">Pleurotus ostreatus</Emphasis> laccase production by culture medium optimization and copper/lignin synergistic induction |
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Authors: | Raunel Tinoco Abisaí Acevedo Enrique Galindo Leobardo Serrano-Carreón |
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Institution: | 1.Instituto de Biotecnología,Universidad Nacional Autónoma de México,Cuernavaca,Mexico |
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Abstract: | Laccases have great biotechnological potential in diverse industries as they catalyze the oxidation of a broad variety of
chemical compounds. Production of laccases by basidiomycetes has been broadly studied as they secrete the enzymes, grow on
cheap substrates, and they generally produce more than one isoenzyme (constitutive and/or inducible). Laccase production and
isoenzyme profile can be modified through medium composition and the use of inducers. The objective of this work was to increase
laccase production by Pleurotus ostreatus CP-50 through culture medium optimization and the simultaneous use of copper and lignin as inducers. Increased fungal growth
was obtained through the use of a factorial fractional experimental design 26–2 where the influence of the nature and concentration of carbon and nitrogen sources was assessed. Although specific laccase
production (U/mg biomass) decreased when malt extract medium was supplemented with carbon and nitrogen sources, fungal growth
and laccase volumetric activity increased four and sixfold, respectively. The effect of media supplementation with copper
and/or lignin on laccase production by P. ostreatus CP-50 was studied. A positive synergistic effect between copper and lignin was observed on laccase production. Overall, the
use of an optimized medium and the simultaneous addition of copper and lignin improved growth, laccase volumetric activity,
and process productivity by 4-, 60-, and 10-fold, respectively. |
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