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Separation of Pneumocystis carinii from the lung of the steriod-suppressed rat
Authors:Mark B. Taylor  Charles S.F. Easmon
Affiliation:Department of Medical Microbiology, St. Mary's Hospital Medical School, London, U.K.
Abstract:Pneumocystis carinii pneumonia was induced in rats by chronic corticosteroid immunosuppression. The parasite was separated from virtually all contaminating host cells using the technique of unit gravity sedimentation. Cellular contamination was routinely below 0.02%. The same technique allowed partial separation of the cyst from the trophozoite stage.
Keywords:Pneumocystis carinii    Speration    Unit gravity sedimentation
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