Intracellular localization of integrin-like protein and its roles in osmotic stress-induced abscisic acid biosynthesis in Zea mays |
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Authors: | B Lü F Chen Z H Gong H Xie J H Zhang J S Liang |
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Institution: | (1) Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou;(2) Biology Department, Hong Kong Baptist College, Kowloon Tang, Hong Kong |
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Abstract: | Summary. Plants have evolved many mechanisms to cope with adverse environmental stresses. Abscisic acid (ABA) accumulates significantly
in plant cells in response to drought conditions, and this is believed to be a major mechanism through which plants enhance
drought tolerance. In this study, we explore the possible mechanisms of osmotic stress perception by plant cells and the consequent
induction of ABA biosynthesis. Immunoblotting and immunofluorescence localization experiments, using a polyclonal antibody
against human integrin β1, revealed the presence of a protein in Zea mays roots that is similar to the integrin proteins of animals and mainly localized in the plasma membrane. Treatment with GRGDS,
a synthetic pentapeptide containing an RGD domain, which interacted specifically with the integrin protein and thus blocked
the cell wall–plasma membrane interaction, significantly inhibited osmotic stress-induced ABA biosynthesis in cells, and the
GRGDS analog which does not contain the RGD domain had no effect. Our results show that a strong interaction exists between
the cell wall and plasma membrane and that this interaction is largely mediated by integrin-like proteins. They also imply
that the cell wall and/or cell wall–plasma membrane interaction plays important roles in the perception of osmotic stress.
Accordingly, we conclude that the cell wall and/or cell wall–plasma membrane interaction mediated by the integrin-like protein
plays important roles in osmotic stress-induced ABA biosynthesis in Zea mays.
Correspondence: J. S. Liang, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, People’s Republic
of China. |
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Keywords: | : Abscisic acid Integrin-like protein Intracellular localization Osmotic stress Zea mays |
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