HMG proteins released from the chromatin following incubation of mammalian nuclei with ethidium bromide |
| |
Authors: | Bianca Pani Paolo Plossi Elio Russo |
| |
Affiliation: | Department of Biochemistry, Biophysics, and Macromolecular Chemistry, University of Trieste, Italy. |
| |
Abstract: | The low-molecular-mass high-mobility group (HMG) chromosomal proteins, namely HMG-14, HMG-17, and HMG-I, which have been found in several proliferating tissues, are released following incubation of nuclei isolated from young rat thymus and from human placenta in a low ionic strength medium containing the intercalating agent ethidium bromide. The amount of HMG proteins released is drug concentration-dependent, but at very high concentrations (20-40 mM) other low- and high-molecular-mass proteins, and even histones, are released. These results suggest a very weak interaction of the HMG proteins with DNA, so that they can be easily detached from the chromatin as a consequence of the interaction of DNA with the intercalating agent. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|