基于灰色关联的苗药红禾麻总黄酮含量影响因子分析

采用Al Cl3比色法对苗药红禾麻42个居群中的总黄酮含量进行分析测定,并用土壤养分测定仪测定对应产地土壤中的铵态氮、速效磷、有效钾、p H值和水分,通过向距产地较近的气象部门查询和以全球卫星定位系统GPS、海拔表等测定地理气候因子,结合42个居群红禾麻ISSR遗传多样性分析结果,运用灰色关联度分析法对红禾麻不同种质资源药材中总黄酮的含量与各影响因子进行相关性分析。结果表明:42个红禾麻不同种质资源药材总黄酮含量为0.42%~2.16%,平均加样回收率为97.86%,RSD为1.5%;环境因子和遗传因子中与总黄酮含量关联度较大的因素分别为无霜期和Shannon信息指数I,而各影响因子与总黄酮含量的关联度中,以Shannon信息指数I(r=1.03)最大,土壤p H(r=0.49)最小,表明遗传因子对红禾麻药材总黄酮含量的影响大于环境因子。该研究结果为红禾麻药材优良种质资源筛选及野生变家种研究提供了依据。

Impact factors of the total flavonoid contents in Guizhou miao medicine Laportea bulbifera based on grey correlation analysis

The total flavonoids content of 42 groups of Guizhou miao medicine Laportea bulbifera were analyzed and determinated by AlCl₃ colorimetric. The ammonium nitrogen, available phosphorus, available potassium, pH and water were determinated in the soil by soil nutrients tester and the origin of geographical climatic factors of L. bulbifera from different producing areas were consulted. In order to analyze the total flavonoids contents of different germplasm resources and impact factors by grey correlation degree analysis, geographical climate factors were determinated by the closed meteorogical department who had been inquired data and GPS, combined with the ISSR results of genetic diversity of 42 groups with the above measured data. The result revealed that the flavonoid contents of L. bulbifera in 42 groups of different germplasm resources was at 0.42%-2.16%, the average recovery rate was 97.86% and RSD was 1.5%, respectively. Frostfree period and the Shannon information index I were the biggest factors with the flavonoid contents in the environment factors and genetic factors. And the biggest correlation degree was Shannon information index I(r=1.03)and the smallest was the soil pH(r=0.49)in all of correlation degrees. It could be judged that genetic factor had more influence on the quality of medicinal materials L. bulbifera than environmental factor. The results provide the reference for choosing good germplasm resources and change the wild to the domestic.