Optimization of fermentation conditions for production of anti-TMV extracellular ribonuclease by Bacillus cereus using response surface methodology |
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Authors: | Wen-Wen Zhou Yun-Long He Tian-Gui Niu Jian-Jiang Zhong |
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Institution: | (1) Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai, 200240, China;(2) College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, Beijing, 100083, China |
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Abstract: | Bacillus cereus ZH14 was previously found to produce a new type of antiviral ribonuclease, which was secreted into medium and active against
tobacco mosaic virus. In order to enhance the ribonuclease production, in this study the optimization of culture conditions
using response surface methodology was done. The fermentation variables including culture temperature, initial pH, inoculum
size, sucrose, yeast extract, MgSO4·7H2O, and KNO3 were considered for selection of significant ones by using the Plackett–Burman design, and four significant variables (sucrose,
yeast extract, MgSO4·7H2O, and KNO3) were further optimized by a 24 factorial central composite design. The optimal combination of the medium constituents for maximum ribonuclease production
was determined as 8.50 g/l sucrose, 9.30 g/l yeast extract, 2.00 g/l MgSO4·7H2O, and 0.62 g/l KNO3. The enzyme activity was increased by 60%. This study will be helpful to the future commercial development of the new bacteria-based
antiviral ribonuclease fermentation process. |
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