节瓜自交系遗传多样性的ISSR分析

利用ISSR分子标记技术对36份节瓜自交系进行遗传多样性分析。从100条ISSR引物中筛选出14条多态性明显、条带清晰、反应稳定的引物,对36份节瓜材料基因组DNA进行扩增,共扩增出76条清晰稳定的条带,其中多态性条带45条,多态性比例为59.21%。36份材料间遗传相似系数在0.57~0.96之间,表明材料间遗传多样性较为狭窄。聚类分析结果显示,以遗传相似系数0.76为阈值时,可将36份节瓜自交系材料聚为3类,分类结果与供试材料的地理来源较为吻合。基于聚类分析结果,可为今后节瓜的新品种选育、遗传改良以及分子遗传连锁图谱构建的杂交亲本选择提供科学依据。

Genetic Diversity of 36 Chieh-qua Inbred lines Based on ISSR

Genetic diversity of 36 Chieh-qua Inbred lines Based on ISSR was analyzed in our research. ISSR markers were used to analyze the genetic diversity of 36 Chieh-qua inbred lines. 14 obviously polymorphic primers were selected from 100 ISSR primers for PCR amplification in 36 Chieh-qua samples. The results showed that 76 bands were amplified from 36 samples, 45 bands were polymorphic and the percentage of polymorphic bands is 59.21%. The genetic similarity coefficient of 36 Chieh-qua samples which ranged from 0.57 to 0.96 showed that genetic diversity was narrow. The cluster analysis showed that 36 Chieh-qua inbred line samples could be classified into three groups, which has a higher correlation with the geographical origin of the samples. The dendrogram could provide a scientific basis for Chieh-qua breeding and parents selection in constructing genetic linkage map of Chieh-qua.