Enhanced expression of various exogenous genes in recombinant Chinese hamster ovary cells in presence of dimethyl sulfoxide |
| |
| Authors: | Chi-Hsien Liu I-Ming Chu Shiaw-Min Hwang |
| |
| Affiliation: | (1) Department of Chemical Engineering, National Tsing-Hua University, Republic of China;(2) Food Industry Research and Development Institute, Hsinchu, Taiwan, 300, Republic of China |
| |
| Abstract: | Dimethyl sulfoxide (DMSO) (1% v/v) stimulated stable transformed Chinese hamster ovary (CHO) cells to synthesize different recombinant proteins and repress their proliferation rate. The expressions of a fusion protein and  -galactosidase were increased 1.6- and 1.4-fold after adding DMSO. The expression of fusion protein was increased by up to 2.8-fold that of uninduced control by the simultaneous addition of DMSO and pentanoic acid. However, DMSO did not increase the production of the monoclonal antibody (immunoglobulin G) of three hybridoma cell lines (OKM1, OKT4 and HyGPD-YK-1-1), although it could inhibit the growth rates of the hybridoma. |
| |
| Keywords: | dimethyl sulfoxide gene expression recombinant mammalian cells |
| 本文献已被 SpringerLink 等数据库收录！ |
