Characterization of monoclonal antibodies directed against distinct conserved epitopes of human immunodeficiency virus type 1 core proteins |
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Authors: | Masashi Tatsumi Christian Devaux François Kourilsky Jean-Claude Chermann |
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Affiliation: | (1) Unité INSERM de Recherches sur les Rétrovirus et Maladies Associeés (U.322), Campus Universitaire de Luminy, B. P. 33, 13273 Marseille Cédex 9, France;(2) CRBM du CNRS, Centre de Tri des Molecules anti-HIV, Boulevard Henri IV, 34060 Montpellier Cedex, France |
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Abstract: | Summary Two mouse hybridoma cell lines secreting antibodies to the Human Immunodeficiency Virus (HIV) p25 major core protein and its precursors p55 and p41, were developed after immunization with the highly cytopathic Zaïrian HIV-1 isolate, NDK. These monoclonal antibodies also react with the gag gene products from HIV-1-BRU prototype and present cross reaction with HIV-2-ROD, and SIV-AGM. They map into topographically distinct areas of p25 and define epitopic regions topographically separated from those recognized by four other anti-p25 mAb suggesting the existence of at least 6 spatially distinct epitopic regions on HIV-1-p25 core protein.Abbreviations HIV Human Immunodeficiency Virus - SIV Simian Immunodeficiency Virus - HTLVI Human T cell Leukaemia Virus - AIDS Acquired Immune Deficiency Syndrome - mAb Monoclonal Antibody - ELISA Enzyme Linked Immunosorbent Assay - PBS Phosphate Buffered Saline |
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Keywords: | human immunodeficiency virus monoclonal antibodies epitope mapping core protein |
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