串珠镰刀菌素降解菌的筛选及特性分析

从黑龙江省镜泊湖附近的草甸土中筛选到一株能以串珠镰刀菌素(MON)为唯一碳源和能源生长的Y21-2菌株。该菌在含500μg/mL MON的基础培养液中菌数从107增长至10１０。根据常规形态特征分析、生理生化性状、G+C mol%含量测定及16S rDNA基因序列分析将其鉴定为根瘤菌科的苍白杆菌属(Ochrobactrum)。静息细胞试验证实Y21-2菌株细胞内确实存在能够降解MON的酶系。

ISOLATION OF MONILIFORMIN-DEGRADING BACTERIUM OCHROBACTRUM SP. AND ANALYSIS OF ITS FUNCTIONAL PROPERTIES

A moniliformin(MON)-degrading bacterium strain, named as Y21-2, was isolated from the mycotoxin-contaminated soil from Heilongjiang Province by the enrichment microculture technique. This strain can grow with MON as its sole carbon and energy source. In the minimal medium with 500 micrograms/mL MON, the number of cells increased from 10(7) to 10(10). Traditional taxonomy, assays of its G + C content and 16S rDNA sequence homology identified Y21-2 as Rhizobiaceae, Ochrobactrum sp. Resting cell suspensions prepared from induced Y21-2 can degrade MON with great speed, which also suggested the existence of enzymes committed to MON degradation in the cell.