| 金属螯合亲和层析纯化金属硫蛋白 |
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| 引用本文： | 铁锋,茹刚,李令媛,刘德富,茹炳根.金属螯合亲和层析纯化金属硫蛋白[J].生物化学与生物物理进展,1994,21(5):447-450. |
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| 作者姓名： | 铁锋 茹刚 李令媛 刘德富 茹炳根 |
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| 作者单位： | 北京大学生物系;北京大学分校生物系;北京大学生物系;北京大学分校生物系;北京大学生物系 |
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| 基金项目： | 国家863(863-103-21-09)资助项目. |
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| 摘 要： | 将二价铜离子螯合在Chelating Sepharose Fast Flow凝胶上制成亲和层析柱,锌诱导兔肝和镉诱导小鼠肝经匀浆、乙醇处理后上柱,用pH4.0的醋酸盐缓冲液平衡,再用pH5.2不同浓度的醋酸盐缓冲液分别洗脱,可得两个金属硫蛋白(MT)洗脱峰,经确定先后为MT-2和MT-1.分离方法比传统的凝胶过滤-离子交换法简单、省时,适于实验室规模分离纯化.
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| 关 键 词： | 金属硫蛋白（MT） 金属螯合 分离纯化 |
| 收稿时间： | 1993/8/17 0:00:00 |
| 修稿时间： | 1993/10/27 0:00:00 |
Purification of Metallothioneins by Metel Chelate Affinity Chromatograpby |
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| Tie Feng,Ru Gang,Li Lingyuan,Liu Defu and Ru Binggen.Purification of Metallothioneins by Metel Chelate Affinity Chromatograpby[J].Progress In Biochemistry and Biophysics,1994,21(5):447-450. |
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| Authors: | Tie Feng Ru Gang Li Lingyuan Liu Defu and Ru Binggen |
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| Institution: | Department of Biology, Peking Uneiversity;Department of Biology, Peking Uneiversity;Department of Biology, Peking Uneiversity;Department of Biology, Peking Uneiversity;Department of Biology, Peking Uneiversity |
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| Abstract: | (China), An affinity chromatography column for isola-tion and purification of metallothionein (MT)was prepared with Chelating Sepharose FastFlow gel bound with bivalent copper. Zinc-in-duced rabbit liver. or cadmium-induced mouseliver was homogenized and precipitated withethanol. The sample was applied on the col-umn and equilibrated with pH4. 0 acetic acidbuffer. Then pH5. 2 of different concentrationacetic acid buffers were used for elution ofMT . Two eluted peaks were obtained andidentified as MT-2 and MT-1. Comparingwith the traditional method -gel filtrationand ion exchange chromatography. thismethod is simple and time-saving in laborato-ry-scale. |
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| Keywords: | metallothionein metal chelating isolation and purification |
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